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Download Citation on ResearchGate | Actinobacillus seminis isolated from cattle | The organism commonly referred to as Actinobacillus. Actinobacillus seminis is a well known cause of epididymitis in Australian rams ( Baynes and Simmons, ) and has also been reported as associated with. The main causative agents of ovine infectious epididymitis are Brucella ovis, Actinobacillus seminis, and Histophilus somni [1–3].

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In contrast, inoculation with H. Thus, considering the importance of ovine infectious epididymitis and the lack of studies relating to infections with A. Validation tests are necessary before the kDa protein can be used in a diagnostic test for ovine epididymitis caused by A.

Subscribe to Table of Contents Alerts. Introduction Contagious ovine epididymitis is defined as infection of the testicle and epididymis in rams. Reports of epididymitis caused by A. To receive news and publication updates for The Scientific World Journal, enter your email address in the box below.

According to previous studies, testicular and epididymal changes caused by inoculation of A. The polypeptide with a molecular mass of 75 kDa identified in the fractions and had an apparently specific immunogenic reaction: The authors declare that there is no conflict of interests regarding the publication of this paper. An invertebrate coagulation system activated by endotoxin: Also, Western-blot testing with serum against whole-cell and field isolates of the same bacteria Table Iobtained from epididymal tissues of rams, revealed that the kDa protein was recognized by serum against isolates of A.

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Microscopic findings in rams experimentally infected with Actinobacillus seminis. For scanning electron microscopy, cultured A. All rams inoculated with A.

They also provide an excellent source for the purification and characterization of biologically active molecules. In all the figures, lane 1 consists of molecular mass markers. This is the first comparative study between A. In most chronic cases, testicular consistency tends to increase, reflecting the atrophy and fibrosis, independent of involved etiologic agent [ 313 ].


For the determination of tissue distribution of agents, fragments of tail, body and head of the epididymes, testicles, ampullae of the vas deferens, seminal vesicles, bulbourethral glands, kidneys, bladder, inguinal and actniobacillus lymph nodes, spleen, and liver were collected. Evidence that extracellular components function in adherence of Actinobacillus actinomycetemcomitans to epithelial cells.

All reagents were from Sigma Chemical Company, St. Bacterial isolates were grouped according to protein profile.

Both groups underwent two months of adaptation and training for semen collection using an artificial vagina. However, the measurements of the left tail of the epididymides i. View at Google Scholar K.

The most severe microscopic lesions were observed at the site of inoculation i. There is a single report of A. Lymphoid hyperplasia was observed in inguinal and iliac lymph node, as well as in the spleen from both experimental groups. Throughout the experimental period, there was no change of scrotal circumference in both experimental groups Figure 1 a.

The values of each point represent the average and standard error. In addition to the epididymis, other organs with marked inflammation in this study included the left seminal vesicles and the vas deferens. Microscopic findings in rams experimentally infected by Histophilus somni. After centrifugation of cultured A. Testicular parameters of rams experimentally infected with Actinobacillus seminis or Histophilus somni during 42 days of infection. The proteins from the 2 isolates had a similar molecular mass.

Furthermore, when each type of hyperimmune serum was tested against the sonicated cells and each of the subcellular fractions of A. The results suggest that the kDa protein could be used to establish a diagnostic test specific for ovine epididymitis caused by A.


No protein of these bacteria cross-reacted with the kDa protein of A.

Actinobacillus seminis (Baynes and Simmons) Sneath and Stevens ATCC &r

Mild multifocal lymphohistiocytic infiltrate was observed in mucosa and submucosa of the urinary bladder. Tsai C, Frasch CE.

Evers cautioned against a test and cull program based on A. View at Google Scholar R. There are rare reports of A. Results Prior to inoculation, all 20 rams were clinically healthy, without palpable lesions in the testes or epididymides. The ewes were running in a pasture paddock in the Orange district of NSW and were supplemented with a mix of grain oats and manufactured sheep pellets. Subclinical cases have been known to persist for up to 4 years.

Urine collection was performed by blocking the breath for 30 seconds. Serum antibody against B. Microscopically, the main lesions were inflammatory changes in the genitourinary tract and testicular degeneration. Ovine epididymitis caused by Actinobacillus seminis.

Actinobacillus abortion

Biological samples obtained from both experimental infections were used to diagnosis proceedings [ 18 ]. Results Protein profiles of A. Ovine infectious epididymitis is one of the major causes of reproductive disorders in sheep, and it strongly impacts on the productivity of the herds [ 1 ].

However, it is still necessary to conduct tests with serum from animals naturally infected with microorganisms other than A. The length and width of the left epididymides tails of rams H.

Ewes are considered to be possible intermediate carriers, promoting ewe-to-lamb transmission 6.


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