ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.
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This method is generally used to substantiate antimicrobial properties of treated specimens for non-public health related claims. Following exposure, a sample of the test organism suspension is removed quantitatively assayed for survivors.
Presence of residual antimicrobial activity indicates the presence of a leaching type of antimicrobial agent. This dynamic e21499 flash test was developed for routine quality control and screening test. One-hour contact time in a buffer solution allows for metabolic stasis in the population.
This test method is designed to evaluate the antimicrobial activity of non-leaching, antimicrobial-treated specimens under dynamic contact conditions. This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism.
It has been developed for routine quality control and screening tests and is used to evaluate substrate bound antimicrobials. It is for this reason that the use of the term leaching throughout this document is 2e149 to only the testing conditions described herein. Work Item s – proposed revisions of this standard. Immobilized antimicrobial agents, such as surface bonded materials, are not free to diffuse into their environment under asttm conditions of use.
Note 1 — Stresses may include laundry, wear and abrasion, radiation and steam sterilization, UV exposure, solvent manipulation, temperature susceptibility, or similar physical or chemical manipulation. Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard.
Microbial concentrations in the treated and untreated samples are usually determined at 0, 1 or 24 hours contact time. The ASTM E Standard Test Method is used to evaluate the antimicrobial activity of substrate-bound, non-leaching, antimicrobial-treated samples under dynamic contact conditions. This standard does not purport to address all of the safety concerns, if any, associated with its use.
The antimicrobial activity of a substrate-bound, non-leaching antimicrobial agent is dependent upon direct contact of microbes with the active chemical agent. Log in Log out Edit. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension. These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately atsm static conditions as in AATCCsensitivity, astn reproducibility.
It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability awtm regulatory limitations prior to use.
All the samples are shaken in dynamic shake d2149 in a wrist action shaker to ensure good contact between the bacteria and the treated fibre, fabric, or other substrate by constant agitation ast the test specimen in a bacterial suspension during the test period.
Summary of Test In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli.
ASTM E – Method Determining the Antimicrobial Activity
Summary of Test Method: A single cookie will be used in your browser to remember your preference not to be tracked.
These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.
Test Package Request Form. No other units of measurement are included in this standard. Work Item s – proposed revisions of this standard. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. The number of viable organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls. This test method is designed to evaluate the resistance of specimens treated with a non-leaching antimicrobial agent to the growth of microbes under dynamic contact conditions.
ASTM E2149 Antimicrobial Activity
Maria Curry ext Wrist Action Shaker. No regulated reduction limits currently exist for general antimicrobial claims made using this method. The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time.